Production of Myxococcus virescens S2-2 alkaline protease by utilizing agro-industrial byproducts: a sustainable alternative for detergent additive and chitin extraction

Background
Myxobacteria are Gram-negative bacteria renowned for their valuable production of secondary metabolites; however, their diverse protease enzymes remain less explored.

Aim
In this investigation, a novel strain was isolated from mangrove soil that produces alkaline protease.

Methods
Using 16S rRNA sequence analysis, the strain was identified as Myxococcus virescens species. The extracellular alkaline protease MvCP of strain S2-2 was produced using growth media containing agro-industrial by-products such as soybean meal and tofu dregs.

Results
MvCP exhibited optimum activity within a pH range from 9 to 10 and temperatures ranging from 30 °C to 50 °C. MvCP activity was improved with Ca2⁺, Mg2⁺, and Zn2⁺ ions, whereas EDTA and β-mercaptoethanol at concentrations of 0.5 and 1.25 mM had no significant effect. MvCP was stable in chloroform, acetone, Triton X-100, Tween-20, and Tween 80.

Conclusion
MvCP can be considered an eco-friendly option for use in household cleaning products and chitin extraction processes.