Snakebite envenoming is an important public health issue with annual mortality rates ranging between 81,000 and 138,000. Snake venoms may cause a range of pathophysiological effects and may have tissue-damaging activities that result in lifelong morbidities. The tissue-damaging components in snake venoms comprise multiple toxin classes with various molecular targets including cellular membranes and the extracellular matrix (ECM). In this study, we present multiple assay formats that enable us to study ECM degradation using a variety of fluorescently labeled ECM components. This workflow could provide valuable insights into the key mechanisms by which proteolytic venom components exert their effects. The workflow could prove useful for the development of effective snakebite treatments.

Released under the CC-BY 4.0 ("Attribution 4.0 International") License

Staff publications

Bittenbinder, M., Bergkamp, Nick D., Slagboom, Julien, Bebelman, Jan Paul M., Casewell, Nicholas R., Siderius, Marco H., … Vonk, F. (2023). Monitoring Snake Venom-Induced Extracellular Matrix Degradation and Identifying Proteolytically Active Venom Toxins Using Fluorescently Labeled Substrates. Biology, 12(6). doi:10.3390/biology12060765