Based on type studies, the following genera are reclassified: Petalosporus, Disarticulatus, and Plunkettomyces as synonyms of Arachniotus; Gymnascella, Gymnoascoides, Macronodus, Tripedotrichum, and Uncinocarpus as synonyms of Gymnoascus, and Kuehniella as a synonym of Arachnotheca. The question of the type species of Arachniotus is discussed. Rollandina is considered to be a nomen confusum; sensu Apinis (1970) it belongs to Nannizzia. The accepted genera of the Gymnoascaceae are briefly reviewed.