In vitro cultivation of malignant lymphoblasts of transplantable Mouse Lymphosarcoma MB (T 86157) without typical mesenchyme cells

Previous investigations (see Literature) by means of tissue culture methods have shown that the malignant lymphoblasts of mouse lymphosarcoma MB (T 86157) can be cultivated indefinitely when in the presence of actively growing mesenchyme cells. Under the cultural conditions provided, which included the use of low concentrations of embryo extract, a symbiotic relationship appeared to be established between the malignant lymphoblasts and the normal mesenchyme. It was also shown that media rich in embryo extract allowed only fibroblasts to proliferate. In the present article we wish to report some results of observations on the cultivation of the malignant lymphoblasts without the supporting mesenchyme.